When samples were not normally distributed or did not show equal variance, Alvocidib order a rank sum test was performed instead. While there was a small decrease in PIC production rates (−11 %), POC quotas and production rates increased strongly under elevated pCO2 (+77 and +55 %, respectively). In conjunction with these changes, the quotas and production rates of TPC also increased (+28 and +23 %, respectively). The PIC:POC ratios of diploid cells decreased from 1.4 to 0.7 under elevated pCO2, while the POC:PON ratios increased from 6.3 to 8.8. Chl a quotas were largely unaffected by the pCO2 treatments, although Chl a:POC ratios decreased significantly from 0.022 to 0.012 pg pg−1 under elevated pCO2, owing to the change in POC quotas. In haploid cells, neither PCI-32765 research buy μ, check details elemental quotas or the respective production rates showed any significant response to elevated pCO2 (Table 3). Similarly, Chl a quotas, Chl a:POC, and POC:PON

ratios were all unaffected by the experimental CO2 manipulations in the haploid strain. Table 3 Growth rates, elemental quotas and production rates, elemental ratios, as well as pigment composition of haploid (1N) and diploid (2N) cells of E. huxleyi, cultured at low (380 μatm) and elevated pCO2 (950 μatm): μ (day−1), POC quota (pg cell−1), POC production (pg cell−1 day−1), PIC quota (pg cell−1), PIC production (pg cell−1 day−1), TPC quota (pg cell−1), TPC production (pg cell−1 day−1), PON quota (pg cell−1), PON production (pg cell−1 day−1), PIC:POC ratio (mol:mol), POC:PON ratio (mol:mol), Chl a quotas (pg cell−1), and Chl a:POC ratios (pg:pg) Parameter 1N low pCO2 1 N high pCO2 p 2N low pCO2 2N high pCO2 p μ 1.12 ± 0.04 1.08 ± 0.06 † 1.08 ± 0.05 1.04 ± 0.04 † POC quota 10.76 ± 0.23 11.08 ± 1.19

† 8.35 ± 0.84 14.78 ± 1.91 ** POC production 12.09 ± 0.25 12.81 ± 0.44 † 9.02 ± 0.91 13.97 ± 0.63 * PIC quota 0.48 ± 0.43 −0.18 ± 0.21 † 11.78 ± 0.78 10.90 ± 0.60 † PIC production – – † 12.71 ± 0.29 11.35 ± 0.90 ** TPC quota 11.23 ± 0.66 12.01 ± 1.27 † 20.13 ± 1.34 25.68 ± 2.00 * TPC production 12.63 ± 0.70 12.51 ± 0.52 † 21.73 ± 1.05 26.77 ± 3.10 ≤ 0.06 PON quota acetylcholine 1.39 ± 0.06 1.45 ± 0.09 † 1.54 ± 0.12 1.95 ± 0.22 * PON production 1.56 ± 0.06 1.56 ± 0.08 † 1.66 ± 0.10 2.03 ± 0.30 † PIC:POC – – † 1.42 ± 0.14 0.75 ± 0.11 ** POC:PON 9.03 ± 0.19 8.90 ± 0.69 † 6.31 ± 0.30 8.83 ± 0.17 *** Chl a quota 0.10 ± 0.01 0.12 ± 0.01 † 0.18 ± 0.01 0.17 ± 0.01 † Chl a OC 0.009 ± 0.001 0.012 ± 0.001 † 0.022 ± 0.001 0.012 ± 0.001 *** For the haploid cells, PIC production and PIC:POC ratios were not calculated.