J Bas Microbiol 2010, 50:119–124. 60. Malone VF, Chastain AJ, Ohlsson JT, Poneleit LS, Nemecek-Marshall M, Fall R: Characterization of a pseudomonas putida allylic alcohol dehydrogenase induced by growth on 2-methyl-3-buten-2-ol. Appl Environ Microbiol 1999,
65:2622–2630.PubMed 61. Sakurai M, Tohda H, Kumagai H, Giga-Hama Y: A distinct type of alcohol dehydrogenase, adh4+, complements ethanol fermentation in an adh1-deficient strain of Schizosaccharomyces pombe. FEMS Yeast Res 2004, 4:649–654.PubMedCrossRef 62. Iijima Y, Wang G, Fridman E, Pichersky E: Analysis of the enzymatic formation of citral in the glands of sweet basil. Arch Biochem Biophys 2006, 448:141–149.PubMedCrossRef 63. Simon R, Priefer U, Puhler A: A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Nat Biotechnol 1983, 1:784–791.CrossRef 64. ABT 263 Schäfer A, Tauch A, Jager W, Kallnowski J, Thierbach G, Pühler A: Small mobilizable multi-purpose cloning vectors derived from the escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of corynebacterium glutamicum. Gene 1994, 145:69–73.PubMedCrossRef 65. Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM, Peterson KM: Four new derivatives of the broad host range cloning vector pBBR1MCS, carrying different antibiotic
resistance cassettes. Gene 1995, 166:175–176.PubMedCrossRef 66. Sambrook J, www.selleckchem.com/products/lcl161.html Russel DW: Molecular cloning: a laboratory manual,
ed 3. Cold Spring Harbor: Cold Spring Harbor laboratory Press; 2001. 67. Inoue H, Nojima H, Okayama H: High efficiency transformation of escherichia coli with plasmids. Gene 1990, 96:23–28.PubMedCrossRef 68. Higuchi R, Krummel B, Saiki RK: A general method of in vitro preparation and specific mutagenesis of Dipeptidyl peptidase DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 1988, 16:7351–7367.PubMedCrossRef 69. Kovach ME, Phillips RW, Elzer PH, Roop RM, Peterson KM: pBBR1MCS: a broad-host-range cloning vector. Biotechniques 1994, 16:800–802.PubMed 70. Bradford MM: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248–254.PubMedCrossRef 71. Biorad: BioRad Protein Assay. Instruction Manual. Munich: BioRad; 1994. 72. Harder J, Probian C: Anaerobic mineralization of cholesterol by a novel type of denitrifying bacterium. Arch Microbiol 1997, 167:269–274.PubMedCrossRef Competing interest The authors declare that they have no competing interests. Authors’ contributions AD isolated the rifampicin resistant C. defragrans strains and assayed the conjugation frequencies. AD constructed pK19mobsacBΔgeoA and obtained C. defragrans ΔgeoA. FL obtained C. defragrans ΔgeoA and Δldi deletion mutants and constructed the pBBR1MCS-2 derivates. FL performed all the physiological experiments.