sebi. Until 2005, the xerotolerant fungal genus Wallemia comprised of a single cosmopolitan species, Wallemia sebi (Zalar et al., 2005). Wallemia sebi is frequently involved in food spoilage of particularly sweet, salty, and

dried food (Samson et al., 2002) and has also often been isolated from indoor and outdoor air (Takahashi, 1997), from soil (Domsch et al., 1990), and from sea salt (DasSarma et al., 2010). Its importance has been further emphasized by its ability to commonly cause allergy problems, which can result in farmer’s lung disease (Lappalainen et al., 1998; Roussel et al., 2004) and cutaneous and subcutaneous infections in humans (De Hoog & Guarro, 1996; Bcl-2 inhibitor review Guarro et al., 2008). As a food-borne mycotoxigenic species, W. sebi isolated from spoiled sweet cake was shown to synthesize mycotoxins walleminol A (Wood et al., 1990) and walleminone (Frank et al., 1999), antitumor antibiotics UCA1064-A and UCA1064-B (Takahashi Ganetespib purchase et al., 1993), and a related, but as yet unidentified, antifungal and cytotoxic metabolite (Mu et al., 2008). To clarify the unresolved phylogenetic position of the genus Wallemia within the fungal kingdom (Wu et al., 2003) and to potentially describe new species within this genus, a large group of strains collected globally were studied. These were obtained from food preserved with low water activity (aw), from different natural hypersaline ecological niches,

and from some medically relevant samples. The morphological, physiological, and molecular characteristics analyzed resolved a new class, Wallemiomycetes, which covers the order Wallemiales (Zalar et al., 2005; Matheny et al., 2006) and includes three species: Wallemia ichthyophaga, W. muriae, and W. sebi. Tests of xerotolerance have shown that the Wallemia spp. represents one of the most xerophilic Demeclocycline fungal taxa (Zalar et al., 2005). However, owing to the previous descriptions related to the complex of species described as W. sebi, the pathogenic and mycotoxin-producing potential of these individual species has remained unknown. Our recent study on the production of bioactive metabolites by different

fungal species that inhabit natural hypersaline environments revealed that organic extracts of all three newly described Wallemia species exert hemolytic activity (Sepčić et al., 2011), which was enhanced at increased salt concentrations. Previous reports on the mycotoxigenic properties of food-borne W. sebi (Wood et al., 1990) and the new finding that an ethanol extract of W. sebi mycelia can induce concentration-dependent hemolysis of red blood cells, thus prompted the present study. As W. sebi can be classified as a serious threat for food safety, the aim here was to investigate hemolytically active extracts of W. sebi in relation to their composition and their specificity toward various lipid membranes and to the effects of external factors. Wallemia sebi EXF-958 (CBS 818.96) originally isolated from sunflower seeds (Zalar et al., 2005) was used.