p-Toluenesulfonate (TSA)

(Fig. 1a) is a xenobiotic arylsulfonate that is widely used in industry and that is found in seepage from landfills (Riediker et al., 2000). Biodegradation of TSA has been explored as a sole source of carbon and energy for bacteria for over 60 years (e.g. Czekalowski & Skarzynski, 1948), and three different pathways have been discovered (Focht & Williams, 1970; Locher et al., 1989; Junker et al., 1994), the best characterized of which is the tsa system in Comamonas testosteroni T-2 (Fig. 1b) (Cook et al., 1999; Providenti et al., 2001; Tralau et al., 2001, 2003a, b; Mampel et al., 2004; Monferrer et al., 2010). The overall objective of this Dapagliflozin manufacturer project was not only to elucidate the enzymatic reactions involved in TSA degradation but also to evaluate their evolutionary origin and potential ecological significance in natural environments. Earlier work showed the world-wide occurrence of TSA degradation, including the tsa operon, but, with one exception, all isolates were from contaminated sites, for

example sewage works: the exception is ‘strain TA12’ from Moorea, an island neighboring Tahiti, French Polynesia (Tralau et al., 2001) – none of the other samples from pristine sites elsewhere in Moorea, in the coastal and marine environments (with varying human impact) of Roscoff (Brittany, France), Carna and Mace Head Co. (Galway, Ireland), Aspropyrgos (Greece) or in the click here pristine peat bog of Murnauer Moos (Bavaria, Germany) yielded any isolates growing on TSA (Tralau et al., 2001). Preliminary analyses of the genomes of C. testosteroni KF1 and Delftia acidovorans SPH1, together with Integrated Microbial Genomes software (http://img.jgi.doe.gov/cgi-bin/pub/main.cgi), indicate the widespread nature of regulons R2 (Wang et al., Mannose-binding protein-associated serine protease 1995, J. Ruff & A.M. Cook, unpublished data) and R4 (Providenti et al., 2001) of the tsa system in Fig. 1b (D. Schleheck & A.M. Cook, unpublished data). Furthermore, an analogue of TSA, p-toluenecarboxylate (TCA), can be considered to occur naturally in turpentine (Cahours, 1850), and the initial reaction steps in the degradation of

TCA involve the same enzymes required for TSA (Junker et al., 1996). At the onset of this study, considerable uncertainty prevailed as to the identity of isolate ‘TA12.’ In order to clarify its taxonomic affiliation and the TSA-degrading pathway of this culture unambiguously, we conducted a combination of reisolations, growth and biochemical experiments as well as sequencing of 16S rRNA genes. Strain TA12’ was obtained in earlier work, and the same complex or carbon-limited salt media were used here (Tralau et al., 2001). Isolated organisms were grown at least as six biological replicates at 28 °C in 150-μL cultures in 300-μL wells of 96-well plates in a plate reader (Synergy HT, Biotek), and all measurements were performed as 10-fold technical replicates.