low-risk patients: optimization using statistical models. Liver Transpl. 2006; 12(2): 231-9. 2. Kaido T, Egawa H, Tsuji H, Ashihara E, Maekawa T, Uemoto S. In-hospital mortality in adult recipients of living donor liver transplantation: experience of 576 consecutive

cases at a single center. Liver Transpl. 2009; 15(11): 1420-5. Clinical features of 450 adult LDLT recipients GRWR, graft-to-recipient weight ratio Disclosures: The following people have nothing to disclose: Murat Dayangac, Murat Akyildiz, Yalcin Erdogan, Gokhan Gungor, Yaman Tokat Introduction: Priming is essential for hepatocytes to proceed and AZD1208 datasheet complete the cell cycle culminating in mitosis and replication. It remains poorly understood how hepatocytes fail to regenerate promptly in elderly animals following a two-third partial hepatectomy (PH). Since γ-aminobutyric acid (GABA) promotes hepatocytes click here into G2 phase of cell cycle, we hypothesized that by priming old hepatocytes

to G2 phase, the liver remnants of old mice regain their regenerative capacity. Methods: We used 24-month (old) and 4-month (young) C57BL/6 mice and evaluated cell cycle distribution by immunohistochemistry for cyclin D1 (G1 phase), cyclin A (S phase), cyclin B1 (G2 phase), Ki67 and pHH3 (M phase). Results: Marked increase of Cyclin B1 positive hepatocytes was seen in aged mice following 7 days of GABA pretreatment, while control mice had mostly quiescent and Cyclin D1 positive cells (Figure 1A). The GABA treated livers regained similar mitotic activity to young controls as seen by pHH3 and Ki67 staining at 36 h after PH. The results were confirmed with western and further explored through gene expression analyses (data not shown). In a separate experiment, mice with and without GABA pre-treatment were followed daily through day 7 post PH to establish cell cycle profile by IHC. We found that Old-GABA mice had proliferative Ki-67 and MCE mitotic pHH3 profiles that were similar to those of young

mice (Figure 1B). Conclusion: Our results indicate that hepatic regenerative capacity after PH in elderly mice can be restored by priming hepatocytes to G2 state prior to PH. Improvement in liver regeneration in elderly will impact quality of liver grafts from elderly donors. Disclosures: The following people have nothing to disclose: Fatima K. Rehman, Toshiyuki Hata, Zhaoyu Li, Guojun Bu, Justin H. Nguyen Introduction: MELD score (Model for End Stage Liver Disease) is universally used to priorities patients on the liver transplant waiting list. It is potentially used to predict survival as well. There has been conflicting evidence on using living donor liver transplantation (LDLT) in patients with high MELD score. We herein showing a retrospective analysis of survival data in these two categories of patients and comparing survival between LDLT and Deceased Donor liver Transplantation (DDLT) in a single center experience.