In the review process, the lack of classification of the main EPS

In the review process, the lack of classification of the main EPS from B. subtilis was noticed. It is often unclear whether a particular polymer under investigation is produced by all wild-type strains of B. subtilis or is unique to a particular isolate. Several hundred wild-type B. subtilis strains have been collected to date, only some of which have the potential to produce different types of EPS. One caveat in these studies is that strains able to secrete polymeric substances are not genetically characterized and those genetically characterized are defective in EPS production. For example, B. subtilis 168 is the most studied type strain, is used in many laboratories and industrial

processes and is an excellent candidate for genetic studies. It is easy to transform, it grows under planktonic conditions, its genome has been sequenced (Kunst et al., 1997) and its proteome has been characterized (Wolff et al., 2007). Unfortunately, B. subtilis 168 produces only a few antibiotics Gefitinib price and it is defective or attenuated in EPS production (Stein et al., 2004; Aguilar et al., 2007). Several of the biosynthetic pathways are not functional because of the domestication processes (i.e. mutations that allow easier genetic manipulations

coupled with repeated growth under artificial settings). The B. subtilis 168 strain derives from X-ray mutations of the original Marburg strain (Burkholder & Giles, 1947; Chu et al., 2006; Earl et al., 2007). In contrast, AZD9291 order various other B. subtilis wild-type strains produce numerous peptide antibiotics as well as abundant EPS (Stein, 2005). In this review, EPS described are specifically matched with the actual Bacillus strains responsible for its production (Table S1). EPS produced by wild-type B. subtilis strains grown under controlled laboratory conditions Thiamine-diphosphate kinase exhibit a wide range of sizes (with molecular weights ranging from 0.57 to 128 kDa) and chemical compositions (i.e. neutral polysaccharides, charged polymers, amphiphilic molecules and proteins) (Priest, 1977; Lin et al., 1999; Omoike & Chorover, 2004). Fourier-transformed infrared

spectroscopy studies of cell-bound and ‘free’ EPS (in aqueous phase) from B. subtilis ATCC7003 grown in Luria broth showed that the composition of the functional groups of the matrix depends on the cell growth phase (e.g. exponential vs. stationary) (Omoike & Chorover, 2004). Greater amounts of free EPS (relative to cell-bound EPS) are produced during the stationary phase. Quantification of the types of macromolecules within these matrices indicated that proteins and carbohydrates are the major constituents of EPS by mass, with protein levels increasing in free EPS as growth proceeded from the exponential to the stationary phase (Omoike & Chorover, 2004). More detailed investigations are needed to explore differences in the abundance and composition of the proteins, acidic groups and sugars of the biofilms of Bacillus grown under specific conditions.

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