Also presented is serotype and phylogenetic groups A, B1, B2 or D. B2 strains are marked with a red box. ColitisI; inactive Ulcerative Colitis, colitisA; active see more Ulcerative Colitis, crohnI, inactive Crohn’s disease, crohnA; active Crohn’s disease. ST; sequence type. Table 2 ExPEC genes in PRIMA-1MET in vitro Escherichia coli isolated from fecal samples from patients with active and inactive IBD and from controls. Disease-Group Reference number Pap A 717 bp afa 594 bp Sfa/foc 410

bp Iut 302 bp kpsM II 272 bp Pap C 205 bp phylogenetic group control c1 – - + – + – B2 control c2 – - + – - – A control c3 – - – - – - D control c4 – - – + – - B1 control c5 – - – - – - B1 control c6 – - – - – - D control c14 – - – - – - B1 control c16 + – - – + – D control c17 – - – - – - A IBDI p10A – - – - – - A   p10B – - – - – - B2 IBDI p11 + – - – + – D IBDI p15 – + – + + + D IBDI p23 – - – - – - A IBDI p26 – - – - – - A IBDI p27 – + – - + – A IBDI p31 – - – - – - B2 IBDI p32 – - + + – - B2 IBDA p7 + + + + – + B2 IBDA p13 – - – - + – B2 IBDA p19A – + + + – + B2   p19B – + – - + – D IBDA p22 + – + + + + B2 IBDA p25 + – + + + + B2 IBDA p29 – - – - – - A IBDA p30 – - – + – + B2 B2 strains with at least one positive ExPEC gene in bold. No verotoxin producing strains were detected among the 26 E. coli isolates examined, and www.selleckchem.com/products/3-methyladenine.html no other common virulence genes were

significantly associated with disease activity based on hybridization assays (table 3). Table

3 Serotype and phenotype of Escherichia coli isolated from fecal samples from patients with active and inactive IBD and from controls. Disease group Reference number Virulence Genes O TYPE K TYPE H TYPE Hemolysin Control c1 – O81 K16 H- – Control c2 astA O6 K39 H- – Control c3 – O77 K96 H18 – Control c4 – O57, O155 K39 H19 – Control c5 – OX184 K- H10 – Control c6 – O126 K- H20 – Control c12 ND         Control c14 – Oru K18 H19 – Control c16 – O1 K1 H- Ent Control c17 astA O101 K+ Pregnenolone H56 – IBD Inactive p10A – O125ac K+ H10 –   p10B – Oru K? H4 – IBD Inactive p11 – O23 K18 H15 Ent. IBD Inactive p15 astA O17 K52 H18 – IBD Inactive p18 ND         IBD Inactive p20 ND         IBD Inactive p23 – O156 K+ H- – IBD Inactive p26 – O9, OX186 K+ H12 Ent. IBD Inactive p27 – O12 K1 H- Ent. IBD inactive p31 – O2 K1 H4 – IBD Inactive p32   O6 K43 H1 – IBD Active p7 astA O2 K2 H1 Ent IBD Active p8 ND         IBD Active p13 – O39 K4 H4 – IBD Active p19A – O6 K2 H1 Alpha   p19B SLM862 O2 K5 H4 – IBD Active p22 – O18ac K5 H- Alpha IBD Active p25 astA O6 K5 H1 Ent. IBD Active p29 aatA O+ K- H10 – IBD Active p30 – O2 K1 H4 – Uropathogenic E. coli associated O-type in bold. Discussion In our study based on fecal samples from patients with previous or present left-sided colitis and from controls, we found a strong correlation between isolation of E.