(C) 2011 American Institute of Physics. [doi:10.1063/1.3633222]“
“Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by extracellular accumulation of amyloid deposits and intracellular neurofibrillary tangles (NFT) composed of hyperphosphorylated Tau proteins. Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor playing a critical role in hippocampal synaptic plasticity and memory and whose levels have been shown reduced in AD brains. While GW4869 mw recent data
support a pivotal role of beta-amyloid peptides towards BDNF decrease, whether Tau pathology impacts on BDNF expression remains unknown so far. In the present study, we have evaluated this relationship using quantitative PCR, Western blot and ELISA in the THY-Tau22 transgenic strain, known to display a progressive development of both hippocampal AD-like Tau pathology and memory impairments. We observed that Tau pathology was not associated
with down-regulation of BDNF at the protein and mRNA levels in this model, suggesting that the alteration of BDNF homeostasis observed in AD patients’ brains might rather be ascribed LDK378 to amyloid pathology.”
“This in vitro study evaluated the roles of diet type and redox potential in the degradation of linalool, (E)- and (Z)- beta-ocimene, alpha-phellandrene, (-)-beta- pinene, (-)-alpha-pinene, (+)-alpha-pinene, sabinene, and alpha-terpinene when incubated with rumen microflora, and PARP inhibitor drugs it provided information on the time course of their disappearance. The 9 monoterpenes are found in the winter
and spring diets of dairy goats in northwestern Mediterranean grazing systems. The diets were individually exposed to rumen microflora for 3 h in 17-mL culture tubes at a concentration of 4 mu L/L. The mixed flora of the inoculum was controlled by the use of vancomycin (eliminating gram-positive bacteria) and by the energy source (starch vs. structural polysaccharides) on which rumen microflora had been grown. Redox potential was controlled by addition of L-cysteine-hydrochloride. The preliminary adaptation of microbial inoculum to a diet rich in structural carbohydrates reduced the recovery yields of (E)- and (Z)-beta-ocimene, (-)-alpha-pinene, (-)-alpha-pinene, (+)-alpha-pinene, and sabinene (P < 0.01), whereas vancomycin was without effect. The effect of carbohydrate source likely stems from the specific composition of the microbial community rather than from its acidogenic capacity. Reducing the culture redox potential by 50 mV reduced the recovery yields of linear and monocyclic terpenes (P < 0.02), and the culture redox potential interacted with the inoculum source for (E)- and (Z)-beta-ocimene and for alpha-phellandrene. The time course of terpene disappearance was studied by exposing terpenes to a rumen microflora adapted either to starch or to fiber for 3, 6, or 24 h.