0 ± 0.6/7.4 ± 0.2/43.7 ± 0.4. After 1 week storage a decrease of CO2 (23-28%) was detected in all packages but after that the gas composition remained essentially the same. Bacterial counts by cultivation during storage Quality of the processed raw material (LS, low salt with 0.4% NaCl) was evaluated upon packaging and the total psychrotrophic load (TVC) was found to contain Saracatinib less than 104 colony forming units (CFU)/g. Initial Pseudomonas spp. load was tenfold lower (Fig. 1) and H2S-producing bacteria almost 100-fold lower than

TVC (data not shown). P. phosphoreum was not detected (< 20 CFU/g) in newly packaged cod loins. Generally, air storage at -2°C did not inhibit bacterial growth compared to storage at 0°C whereas storage at -4°C clearly showed a reduced growth throughout the storage time (Fig. 1 and 2). In MAP fish, storage temperature clearly influenced bacterial growth, with an increased delay as temperature decreased. Monitoring of P. phosphoreum showed a reduction in growth with lower temperatures, especially when combined with MA (Fig. 1). Figure 1 Bacterial growth in air and MA cod loins (LS). Bacterial growth in air- and MA-packaged cod loins (LS)

during storage at A) 0°C, B) -2°C and C) -4°C. (black square) Total psychrotrophic viable counts in MA, (white square) total psychrotrophic viable counts in air, (black circle) presumptive Pseudomonas counts in MA, (white circle) presumptive Pseudomonas counts in air,

(black triangle) P. phosphoreum in MA and (white triangle) P. phosphoreum in air. Figure 2 Bacterial growth in Ibrutinib in vitro air and MA cod loins (HS). Bacterial growth in air- and MA-packaged cod loins (HS) during storage at A) -2°C and B) -4°C. (black square) Total psychrotrophic viable counts in MA, (white square) total psychrotrophic viable counts in air, (black circle) presumptive Pseudomonas counts in MA, (white circle) presumptive Pseudomonas counts in air, (black triangle) P. phosphoreum in MA and (white triangle) P. phosphoreum in air. Pseudomonas also spp. showed an increasing growth during storage in air, both at 0 and -2°C, but with some delay at -4°C. MAP had a biostatic effect on pseudomonads development, resulting in constant counts (between 3 and 4 log10 CFU/g) at all temperatures. Similar trends could be seen during storage of brined (HS, high salt with 2.5% NaCl) fish where combining MA and lower temperature storage generally inhibited bacterial growth (Fig. 2). Relative ratio of selected spoilage organisms showed a large variation of dominance. Pseudomonas spp. were usually in high proportional concentrations during air storage (up to 58.9%) and at lower concentrations during MA storage. However, on day 7 at -4°C in MA storage, Pseudomonas spp. reached a level of 33% of the flora in both the LS and HS groups. P. phosphoreum was at low relative concentrations (0 – 6%) except during MA storage at 0°C where it reached up to nearly 100% (Table 1).