Isolation, characterization, and evidence for the existence of complexes with hemagglutinins. The Journal of biological chemistry 1994,269(1):406–411.PubMed 26. Potempa J, Mikolajczyk-Pawlinska J, Brassell D, Nelson D, Thogersen IB, Enghild JJ, Travis J: Comparative properties of two cysteine proteinases (gingipains

R), the products of two related but individual genes of Porphyromonas gingivalis. The Journal of biological chemistry 1998,273(34):21648–21657.CrossRefPubMed 27. Potempa J, Nguyen KA: Purification and characterization of gingipains. Current protocols in protein science/editorial board, John E Coligan [et al] 2007,Chapter 21(Unit 21):20. 28. Potempa J, Pike R, Travis J: Titration Selleckchem AZD1152 and mapping of the active site of cysteine proteinases from Porphyromonas gingivalis (gingipains) using peptidyl chloromethanes. Biol Chem 1997,378(3–4):223–230.CrossRefPubMed 29. Kinane DF, Shiba H, Stathopoulou PG, Zhao H, www.selleckchem.com/products/icg-001.html Lappin DF, Singh A, Eskan MA, Beckers S, Waigel S, Alpert B, et al.: Gingival epithelial cells heterozygous for Toll-like receptor 4 polymorphisms Asp299Gly and Thr399ile are hypo-responsive to Porphyromonas gingivalis. Genes and immunity 2006,7(3):190–200.CrossRefPubMed Competing interests The authors declare that they have no competing interests. Authors’ contributions

DFK, JCG and PGS designed the study and drafted the manuscript. PGS carried out majority of the experiments. JCG carried out the apoptosis assays. MRB designed the PCR array experiments and helped in drafting the manuscript. CAG carried out the flow cytometry experiments. JP provided 20s Proteasome activity critical comments to improve the manuscript. All authors were involved in analyzing all the data, read and approved the final manuscript.”
“Background Geobacter metallireducens is a member of the Geobacteraceae, a family of Fe(III)-respiring Delta-proteobacteria

that are of interest for their role in cycling of carbon and metals in aquatic sediments and subsurface environments not as well as the bioremediation of organic- and metal-contaminated groundwater and the harvesting of electricity from complex organic matter [1, 2]. G. metallireducens is of particular interest because it was the first microorganism found to be capable of a number of novel anaerobic processes including: (1) conservation of energy to support growth from the oxidation of organic compounds coupled to the reduction of Fe(III) or Mn(IV) [3, 4]; (2) conversion of Fe(III) oxide to ultrafine-grained magnetite [3]; (3) anaerobic oxidation of an aromatic hydrocarbon [5, 6]; (4) reduction of U(VI) [7]; (5) use of humic substances as an electron acceptor [8]; (6) chemotaxis toward metals [9]; (7) complete oxidation of organic compounds to carbon dioxide with an electrode serving as the sole electron acceptor ([10]; and (8) use of a poised electrode as a direct electron donor [11].